Prion



Prion (PrP) is a protein which becomes infectious upon undergoing conformation change to an amyloid form, which is self-propagating and becomes resistant to protease degradation. The fungus Podospora anserine has a prion-like protein HET-S which undergoes a conformation change to amyloid form which prevents its colony from merging with non-compatible colonies. Yeast prion proteins are Sup35 and Ure2. The images at the left and at the right correspond to one representative prion, i.e. the crystal structure of human prion (2haf).

3D structures of prion
Update June 2011

PrP short polypeptides
3nve – ShPrP residues 138 -143 – Syrian hamster

2kkg - PrP residues 23 -106 – Golden hamster - NMR

3nvf – hPrP residues 138 -143 – human

2ol9 - hPrP residues 170 – 175

3nhc, 3nhd, 3md4, 3md5 - hPrP residues 127 – 132

2iv5 - hPrP residues 173 -195 – NMR

1oei - hPrP residues 61 - 84 – NMR

1oeh - hPrP residues 61 - 68 – NMR

2iv6 - hPrP residues 173 -195 (mutant) – NMR

2iv4 - hPrP residues 180 -195 – NMR

3nvg, 3nvh - mPrP residues 138 -143 – mouse

1skh - bPrP residues 1 – 30 - bovine

3fva - ePrP residues 173 -178 – Elk

1s4t - sPrP residues 135 – 155 – sheep – NMR

1m25 - sPrP residues 152 – 156 – NMR

1g04 - sPrP residues 145 – 169 – NMR

2rmv, 2rmw - sPrP residues 142 – 166 (mutant) – NMR

PrP
3o79 – rPrP C-terminal – rabbit

2fj3 - rPrP C-terminal – NMR

2joh, 2jom - rPrP C-terminal (mutant) – NMR

1xyw – ePrP C terminal - NMR

2ku4 - PrP C-terminal – horse

3fva - ePrP C-terminal – NMR

2kfl - PrP C-terminal – Wallaby – NMR

2k56 - PrP C-terminal – Vole – NMR

2ktm – sPrP residues 167-234 H2H3 domain (mutant) – NMR

1xyu, 1y2s - sPrP C-terminal – NMR

1uw3 - sPrP C-terminal

3haf, 3hak, 3hj5, 1i4m - hPrP C-terminal

1hjm, 1hjn, 2kun – hPrP C-terminal – NMR

1h0l, 1fkc, 2k1d, 1fo7, 1e1s, 1e1g, 1e1j, 1e1p, 1e1u, 1e1w, 1qlx, 1qlz, 1qm0, 1qm1, 1qm2, 1qm3, 1qlz, 1qm0, 1qm1- hPrP C-terminal (mutant) - NMR

3heq, 3her, 3hes, 3hjx - hPrP C-terminal (mutant)

2ku5, 2ku6, 2kfm, 2kfo, 2k5o, 1y16, 1y15 - mPrP C-terminal (mutant)

1xyx - mPrP C-terminal

1ag2 - mPrP residues 121-231 - NMR

1u3m – PrP C-terminal – chicken – NMR

1u5l - PrP C-terminal – turtle – NMR

1xu0 - PrP C-terminal – frog – NMR

1xyj - PrP C-terminal – cat – NMR

1xyk - PrP C-terminal – dog – NMR

1xyq - PrP C-terminal – pig – NMR

1dwy, 1dx0, 1dx1 - bPrP C-terminal – NMR

1dwz - bPrP residues 121-230 (mutant) - NMR

1b10 - ShPrP C-terminal – NMR

Yeast prions
2onx, 2olx – Sup35 residues 8 - 11 – yeast

2omm, 1yjo, 1yjp – Sup35 residues 7 – 13

1jzr, 1k0a, 1k0b, 1k0c, 1k0d – Ure2p + glutathione derivartive

1g6w, 1g6y – Ure2p globular domain

1hqo – Ure2p nitrogen regulation fragment

PrP+antibody
2w9e - hPrP C-terminal + anti-PrP antibody

2hh0 - bPrP peptide epitope + anti-PrP antibody

1cu4 - ShPrP peptide epitope + anti-PrP antibody

1tpx, 1tqb, 1tqc - sPrP C-terminal + anti-PrP antibody

HET-S from Podospora anserine
2kj3, 2rnm – HET-S C-terminal – NMR

2wvn, 2wvo - HET-S N-terminal

2wvq - HET-S N-terminal (mutant)